军团菌诊断血清（凝集法）

广州健仑生物科技有限公司

广州健仑长期供应：军团菌、诺如病毒、流感病毒等传染病系列的快速检测试剂盒。

军团菌的检测试剂盒包括：军团菌尿液抗原检测试剂盒、军团菌抗体快速检测卡（胶体金法）、军团菌抗原快速检测卡（胶体金法）、军团菌水样检测试剂盒、军团菌乳胶凝集试剂盒（军团菌诊断血清）、嗜肺军团菌核酸荧光PCR检测试剂盒。

我司还提供其它进口或国产试剂盒：包括传染病系列、免疫组化系列、诊断血清等产品欢迎致电。

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【日本生研军团菌诊断血清（凝集法）】

200549 军团菌诊断血清  套装 2ml  10支

214997        　pneumophila１群    嗜肺军团菌 2ML

215000        　pneumophila２群 规格：2ML

215017        　pneumophila３群 规格：2ML

215024        　pneumophila４群 规格：2ML

215031        　pneumophila５群 规格：2ML

215048        　pneumophila６群 规格：2ML

215055          bozemanii   博兹曼军团菌 2ML

215062          dumoffii    杜莫夫军团菌    2ML

215079          gormanii    戈尔曼军团菌 2ML

215086          micdadei    米克戴德军团菌 2ML

215727        　pneumophila7群 规格：2ml

215734        　pneumophila8群 规格：2ml

293572        　pneumophila9群 规格：2ml

293589        　pneumophila10群 规格：2ml

293626        　pneumophila11群 规格：2ml

293633        　pneumophila12群 规格：2ml

293640        　pneumophila13群 规格：2ml

293657        　pneumophila14群 规格：2ml

293664        　pneumophila15群 规格：2ml

【日本生研军团菌诊断血清（凝集法）】

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【公司名称】 广州健仑生物科技有限公司

【市 场 部】 13802525278 020-82574011  杨永汉

【公司

【腾讯Q Q】 2042552662

【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

1. 细菌培养法
军团菌为革兰氏阴性杆菌，专性需氧，胞内寄生菌。军团菌最初是从以军团菌病人肺组织感染的豚鼠中分离出来的。这种方法虽然有较高的可靠性，但非常昂贵，而且费时费力，不久就被平板培养法所取代。目前标准培养基为活性碳酵母浸膏琼脂平板，也称军团菌生长平板(BCYE)。接种在 BCYE 平板上的样品在温度为35-37oC，培养10天，在浓度为2.5% CO2 的环境下培养更有利于军团菌的生长。军团菌的菌落通常呈白色、灰色、有荧光。军团菌在 BCYE 平板上生长而在平板和半光氨酸缺失 BCYE-Cys 平板上不生长。
2.血清学玻片凝集法
军团菌感染l周左右可检测出血清中军团菌特异性IgM抗体，2周左右可检测到特异性IgG抗体。
现已发现嗜肺军团菌有15个血清型。其中，嗜肺军团菌血清1型（Lp1）与人类疾病关系最密切，其次为血清4型（Lp4）和6型（Lp6）。
3.核酸扩增法
核酸扩增技术，即聚合酶链式反应（polymerase chainreaction，PCR），其基本原理是设计、合成两条寡核苷酸，作为引物，对应于待测病原微生物某一段特异性序列的两端，然后在体外模拟DNA体内复制的过程反复扩增，使靶序列放大上万倍甚至上百万倍而被检测出来。
常规PCR：鉴定标准是通过电泳来判断是否有扩增的核酸片段以及扩增产物的大小是否正确。
实时荧光PCR：通过对实时荧光PCR反应的每一个循环产物进行荧光信号的实时监测来判断分析。

Bacterial culture
Legionella is Gram-negative bacilli, obligate aerobic, intracellular parasites. Legionella was first isolated from guinea pigs infected with lung tissue of Legionnaires' patients. Although this method has high reliability, but very expensive, but also time-consuming and laborious, and soon was replaced by plate culture method. The current standard medium is activated carbon yeast extract agar plate, also known as Legionella growth plate (BCYE). The samples inoculated on BCYE plates grew at a temperature of 35-37oC for 10 days and cultured at a concentration of 2.5% CO 2 more conducive to Legionella growth. Legionella colonies are usually white, gray, fluorescent. Legionella grew on BCYE plates and did not grow on plates and half-my-leucine-deficient BCYE-Cys plates.
2. Serological slide agglutination method
Legionella infection can be detected about 1 week in the serum of Legionella specific IgM antibodies, about 2 weeks to detect specific IgG antibodies.
Legionella pneumophila has been found in 15 serotypes. Among them, Legionella pneumophila serotype 1 (Lp1) has the closest relationship with human diseases, followed by Lp4 and Lp6.
3 nucleic acid amplification method
The principle of nucleic acid amplification, that is, polymerase chain reaction (PCR), is to design and synthesize two oligonucleotides, which serve as primers and correspond to the two ends of a specific sequence of the pathogenic microorganism to be tested. Then in vitro replication of DNA replication in vivo repeated amplification process, the target sequence to enlarge tens of thousands or even millions of times were detected.
Conventional PCR: The standard of identification is determined by electrophoresis to determine whether the amplified nucleic acid fragment and the size of the amplified product are correct.
Real-time fluorescence PCR: Real-time fluorescence PCR reaction through real-time monitoring of each signal to determine the fluorescence signal analysis.